|                          Time                         (min)                          |                                                  Flow                         (ml/min)                          |                                                  A%                          |                                                  B%                          |                                                                  |                          0                          |                                                  1ml/min                          |                                                  65                          |                                                  35                          |                                                                  |                          15                          |                                                  65                          |                                                  35                          |                                                                  |                          20                          |                                                  55                          |                                                  45                          |                                                                  |                          26                          |                                                  55                          |                                                  45                          |                                                                                           |         
    
Column: Amethyst C18-P (5um, 4.6×150mm, 120Å SN:09100918716)
 phase: A: 戊烷磺酸鈉1.5g+磷酸二氫銨3.5g+水950ml,用磷酸調(diào)pH=3.0,用水稀釋至1000mL
B:甲醇
Flow rate: 1.0mL/min Column Temp: 25℃ Wavelength: 287nm
Injection Volume: 10ul                Pressures: 170.5~171.5Bar
Instrument:Agilent1200 自動(dòng)進(jìn)樣